Anti-MK5/MAPKAPK5 antibody (192-206) (STJ72941)-Anti-MK5/MAPKAPK5 antibody (192-206) (STJ72941)-一抗检测抗体

: Anti-MK5/MAPKAPK5 antibody (192-206) (STJ72941)

Anti-MK5/MAPKAPK5 antibody (192-206) (STJ72941)

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品牌介绍：: Stjohnslabs为生物技术、制药和学术界的生命科学家提供研究试剂盒、抗体和试剂的专业供应商。我们的平台为蛋白质和代谢物的检测、鉴定和定量提供了广泛的研究材料。

其他：: 克隆性：Polyclonal
浓度：0.5 mg/mL
稀释范围：Peptide ELISA: antibody detection limit dilution 1:64000.
WB: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1µg/ml.
IHC: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8µg/ml.
IP: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of the IP were labelled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.