Anti-MK2/MAPKAPK2 antibody (Internal) (STJ72942)-Anti-MK2/MAPKAPK2 antibody (Internal) (STJ72942)-一抗检测抗体

: Anti-MK2/MAPKAPK2 antibody (Internal) (STJ72942)

Anti-MK2/MAPKAPK2 antibody (Internal) (STJ72942)

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品牌介绍：: Stjohnslabs为生物技术、制药和学术界的生命科学家提供研究试剂盒、抗体和试剂的专业供应商。我们的平台为蛋白质和代谢物的检测、鉴定和定量提供了广泛的研究材料。

其他：: 克隆性：Polyclonal
浓度：0.5 mg/mL
稀释范围：Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 60kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kDa according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec;66 (12) :1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.
IP: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2 (CST). An approx 45kDa of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.