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CFSE Green Fluorescent Stain - for cell labelling and proliferation studies
CFSE Green Fluorescent Stain - for cell labelling and proliferation studies
- 中文名称：
- CFSE Green Fluorescent Stain - for cell labelling and proliferation studies
- 英文名称：
- CFSE Green Fluorescent Stain - for cell labelling and proliferation studies
- 品牌：
- Immuno Chemistry
- 货号：
- 6162
- 规格：
- 50 ug
- 保存建议：
- -20°C
- 背景资料：
- CFSE is a green fluorogenic reagent that binds to intracellular molecules. It is often used for cell proliferation and cytotoxicity studies. CFSE diffuses into the cell and covalently binds to primary amino groups present on intracellular molecules. Intracellular esterases quickly cleave the acetate groups from the dye thus converting it to the fluorescent form. Any unbound reagent diffuses back out of the cell. Because CFSE forms a strong bond inside the cell, it is retained within the cell indefinitely and is inherited by daughter cells. It will not be incorporated into adjacent cells.CFSE is supplied as a concentrated lyophilized powder at 0.05 mg. Reconstitute it with 200 µL DMSO to yield a stock concentrate at 2500X (0.25 mg/mL). Dilute it 1:250 in PBS to form the 10X working solution, and then add it to cells at 1:10 (a final concentration of 0.1 µg/mL). Analyze with a fluorescence microscope or flow cytometer with a 488 nm blue argon excitation laser. CFSE exhibits green fluorescence in the FL1 region: excitation at 492 nm and emission at 520-540 nm (Figures 1-3).As CFSE is detected in the green range, it is optimal for use in dual- staining studies with other fluorescent reagents, such as PI (catalog #638), 7-AAD (catalog #6163), and SR-FLICA® reagents with minimal spectral overlap. It can be used with our SR-FLICA® poly caspases inhibitor reagent (catalog #917) to identify apoptotic cells in the cell sample (Figure 3).
- 货期：
- 4-6周
- 检测方法：
- Flow cytometry, Fluorescence microscope
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
CFSE is a fluorogenic reagent which is frequently used in cell labeling and cell proliferation procedures. Non-fluorescent and moderately hydrophobic in its di-esterified form, the dye easily penetrates lipid bi-layers of living cells and is quickly converted to the amine-reactive, green fluorescent form (CFSE) upon cleavage of the acetate groups by intracellular esterases. This highly fluorescent form is membrane-impermeant and is retained on and within the cells, while any excess probe is easily washed away in subsequent wash steps. Because CFSE forms a strong bond inside the cell, it is retained within the cell indefinitely and is inherited by daughter cells. It will not be incorporated into adjacent cells.
