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7-AAD Red Fluorescent Live/Dead Stain
7-AAD Red Fluorescent Live/Dead Stain
- 中文名称：
- 7-AAD Red Fluorescent Live/Dead Stain
- 英文名称：
- 7-AAD Red Fluorescent Live/Dead Stain
- 品牌：
- Immuno Chemistry
- 货号：
- 6163
- 规格：
- 0.26 mg
- 保存建议：
- 2-8°C
- 背景资料：
- 7-Aminoactinomycin D (7-AAD, catalog 163) is an intercalating red fluorescent reagent that binds between cytosine and guanine bases of DNA in membrane-compromised cells. This material, like its parent molecule, Actinomycin D, is a DNA-intercalator with growth-inhibitory properties. Normal healthy cells, with intact membrane structure, will exclude the polar 7-AAD vital dye. Mid to late apoptotic and necrotic cells will not exclude this dye and subsequently stain red when 7-AAD complexes with the nuclear DNA. As 7-AAD is membrane impermeant, it cannot reach the DNA in viable cells, thus allowing the identification of cells with permeabilized membranes in a population. 7-AAD distinguishes between living and dead cells by counterstaining nucleic acids red in necrotic, dead, dying, and membrane-compromised cells, while the DNA in healthy cells remains unstained.7-AAD is supplied as a highly concentrated lyophilized powder at 0.26 mg/vial. Reconstitute it with 260 µL DMSO to yield a stock concentrate at 1 mg/mL. Add it to the cells at a final concentration of 5 µg/mL and analyze with a flow cytometer (Figure 1) or fluorescence microscope (Figure 2). When bound to nucleic acids, the maximum absorption is 546 nm and the maximum emission is 647 nm.7-aminoactinomycin D may be used in combination with other fluorescent reagents for dual staining or multiplexing purposes. Use it with our FAM-FLICA® poly caspases inhibitor reagent (catalog 637) to include apoptotic cells in the analysis. When used together, these reagents will identify four populations of cells: living; early apoptotic; late apoptotic; and necrotic cells leading to more accurate results when assessing cell death (Figures 2 and 3).
- 货期：
- 4-6周
- 检测方法：
- Flow cytometry, Fluorescence microscope
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
7-Aminoactinomycin D (7-AAD) is a red fluorescent vital dye that intercalates in double-stranded DNA with a high affinity for GC-rich regions. 7-AAD does not pass through intact cell membranes. It may also be used to label all cells after fixation.
