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aCella - Tox - an assay designed to measure cell cytoxicity
aCella - Tox - an assay designed to measure cell cytoxicity
- 中文名称：
- aCella - Tox - an assay designed to measure cell cytoxicity
- 英文名称：
- aCella - Tox - an assay designed to measure cell cytoxicity
- 品牌：
- Immuno Chemistry
- 货号：
- CLATOX100-3P
- 规格：
- 500 Tests (with 5 Lumi Plates + 5 TC Plates)
- 保存建议：
- Store contents as labeled
- 背景资料：
- GAPDH is an important enzyme in the glycolysis and gluconeogenesis pathways. This homotetrameric enzyme catalyzes the oxidative phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate in the presence of cofactor and inorganic phosphate. In the aCella-TOX reaction scheme the release of GAPDH is coupled to the activity of the enzyme 3-Phosphoglyceric Phosphokinase (PGK) to produce ATP. ATP is detected via the luciferase, luciferin Bioluminescence methodology. Further, aCella-TOX is a homogeneous cytotoxicity assay; alternatively in dual mode, aCella-TOX can measure cytotoxicity and cell viability in the same plate. Culture supernatants can also be removed from the original plate and assayed in a different plate, allowing kinetics runs to be set up. The assay is non-destructive, allowing the monitoring of additional parameters such as gene expression.
- 货期：
- 4-6周
- 检测方法：
- Luminometer
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells.
