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Adenine Nucleotide Translocator 2 Antibody
Adenine Nucleotide Translocator 2 Antibody
- 中文名称：
- Adenine Nucleotide Translocator 2 Antibody
- 英文名称：
- Adenine Nucleotide Translocator 2 Antibody
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA75808
- 规格：
- 0.1 mg|2x0.1 mg|3x0.1 mg|4x0.1 mg|5x0.1 mg
- 保存建议：
- -20°C for long term storage
- 货期：
- 6-8周
- 来源宿主：
- Rabbit
- 反应种属：
- Human, Mouse, Rat
- 应用：
- WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, CM (Confocal Microscopy)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Polyclonal
同型:N/A
克隆号:N/A
特异性:N/A
纯度:N/A
形式：One hundred micrograms (100ug) of antibody stabilization buffer.
浓度：0.75 mg/ml in antibody stabilization buffer
- 说明书：
Affinity Purified ADP/ATP Translocase 2 Antibody.
Overview: Human Adenine Nucleotide Translocator (ANT) presents four isoforms (ANT1-ANT4), each with a specific expression depending on the nature of the tissue, cell type, developmental stage and status of cell proliferation. ANT1 is specific to muscle and brain tissues, ANT2 occurs mainly in proliferative, undifferentiated cells, ANT3 is ubiquitous, and ANT4 is found in germ cells. ANT2 is a homodimer and a component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. ANT2, also known as solute carrier family 25 mitochondrial carrier member 5 (SLC25A5, is a 298 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and contains three solcar repeats. ANT2 functions as a homodimer that facilitates the exchange of ADP and ATP between the mitochondrion and the cytosol, thus linking the compartment of ATP production (within the mitochondrion) to the areas of ATP utilization (within the cytosol). ANT2 expression in undifferentiated, proliferating cells correlates with the rate of glycolytic metabolism and is an indicator of carcinogenesis. Suppression of ANT2/SLC25A5 expression by specific RNA interference in human breast cancer cells promotes apoptosis and inhibits tumor cell growth which suggest a cytoprotective role of ANT2/SLC25A5. ANT2 functions differently in metabolic and apoptotic pathways and is implicated in the function of the permeability transition pore complex (PTPC), which regulates the release of mitochondrial products that induce apoptosis.
As part of the mitotic spindle-associated MMXD complex, ANT2 plays a role in chromosome segregation, and it is also known to interact with HIV-1 Vpr protein. ANT2 functions in a similar manner to ANT3 but, unlike ANT3, it also plays a role in the formation of the permeability transition pore complex (PTPC), a structure that is important for the regulation of apoptosis. The expression of ANT2 is linked to and is an important indicator of carcinogenesis. Since the expression of ANT2 is closely linked to the rate of glycolytic metabolism and to the mitochondrial bioenergetics of tumors, it should be taken into account for individualizing cancer treatments and for the development of anticancer strategies. The human genome contains several non-transcribed pseudogenes of this gene. The role of ANT2 as an endogenous inhibitor of MMP (Mitochondrial membrane permeabilization) suggests that its selective inhibition could constitute a promising strategy of chemosensitization.
ANT2-selective antibodies were generated against a peptide taken from mouse and human ANT2 protein. The ANT2-selective antibodies are affinity purified on an immobilized antigen based affinity matrix and the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The ANT2-selective antibodies are fully characterized for applications in western blot and ELISA at the recommended dilutions. Antigenic blocking peptides (P-ANT2) and western blot positive control (PC-ANT2) in ready to use SDS-sample buffer are available. Primary antibody ANT2- 201AP labels a ~36 kDa protein in PC-ANT2 samples and several other tissues. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost
