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GAP43
GAP43
- 中文名称：
- GAP43
- 英文名称：
- GAP43
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA76708
- 规格：
- 0.1 mL|2x0.1 mL|3x0.1 mL|4x0.1 mL|5x0.1 mL
- 保存建议：
- The antibody can be stored at 2 degree - 8 degree C for 12 months without detectable loss of activity. Avoidrepeated freeze-thaw cycles.
- 货期：
- 6-8周
- 来源宿主：
- Chicken
- 反应种属：
- Human, Mouse, Rat, Bovine, Porcine, Equine
- 应用：
- IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Polyclonal
同型:Chicken IgY
克隆号:N/A
特异性:N/A
纯度:N/A
形式：Liquid. Supplied as an aliquot of IgYpreparation plus 5mM NaN3
浓度：N/A
- 说明书：
GAP43 is an abundant protein which is found heavily concentrated in developing neurons, in particular at the growing tips, the growth cones. One group discovered it since it becomes unregulated during the regeneration of the toad optic nerve, and named it "growth associated protein 43." GAP43 is very highly charged and does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, since human GAP43 is 238 amino acids giving a real molecular weight 24.8kDa. The same GAP43 preparation will also give a different SDS-PAGE molecular weight depending on the percentage acrylamide content of the gel, the protein appearing relatively larger on gels with higher acrylamide concentration. GAP43 proteins from different species also may run at different apparent molecular weights on the same gel. Partly due to these unusual features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57, the numbers 46, 50 and 57 reflecting the apparent SDS-PAGE molecular weight. GAP43 is a major protein kinase C substrate and binds calmodulin avidly, this being mediated by an N-terminal IQ calmodulin binding motif. GAP43 may be anchored to the plasma membrane by reversible palmitoylation on two Cys residues close to the N-terminus. Knock out of the GAP43 gene in mice is lethal early in postnatal life and is associated with defects in axonal pathfinding. GAP43 is one of a large family of "intrinsically disordered proteins" which typically have little defined structure unless they are bound to a more structured partner.
This antibody was made against the full length recombinant human protein and binds to GAP43 in rodents and other mammalian species. It binds strongly to growth cones and axonal processes of neurons in cell culture and to synaptic regions in sectioned material.
