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Anti-HSD11B2 Antibody Picoband
Anti-HSD11B2 Antibody Picoband
- 中文名称：
- Anti-HSD11B2 Antibody Picoband
- 英文名称：
- Anti-HSD11B2 Antibody Picoband
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA19425
- 规格：
- 0.01 mg|0.1 mg|0.1 mg (Carrier Free)|0.1 mg (HRP)|0.1 mg (FITC)
- 保存建议：
- Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
- 货期：
- 6-8周
- 来源宿主：
- Rabbit
- 反应种属：
- Human
- 应用：
- WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Polyclonal
同型:Rabbit IgG
克隆号:N/A
特异性:Natural and recombinant Human CD27
纯度:Immunogen affinity purified.
形式：Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
浓度：Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
- 说明书：
Background: CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Principle of the Assay: The Quick Picokine™ Human CD27 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD27 in cell culture supernatants and serum. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human CD27 with immunogen: Expression system for standard: NS0; Immunogen sequence: T21-I192. To measure Human CD27, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CD27 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD27 in the sample.
