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Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband
Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband
- 中文名称:
- Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband
- 英文名称:
- Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA19426
- 规格:
- 0.01 mg|0.1 mg|0.1 mg (Carrier Free)|0.1 mg (HRP)|0.1 mg (FITC)
- 保存建议:
- Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
- 货期:
- 6-8周
- 来源宿主:
- Rabbit
- 反应种属:
- Human
- 应用:
- ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 其他:
克隆性:Polyclonal
同型:Rabbit IgG
克隆号:N/A
特异性:Natural and recombinant Human CD40
纯度:Immunogen affinity purified.
形式:Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
浓度:Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
- 说明书:
Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Principle of the Assay: The Quick Picokine™ Human CD40 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD40 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human CD40 with immunogen: Expression system for standard: NS0; Immunogen sequence: E21-R193. To measure Human CD40, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CD40 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD40 in the sample.