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S-Tag/S15
S-Tag/S15
- 中文名称：
- S-Tag/S15
- 英文名称：
- S-Tag/S15
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA76728
- 规格：
- 0.1 mL|2x0.1 mL|3x0.1 mL|4x0.1 mL|5x0.1 mL
- 保存建议：
- Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 货期：
- 6-8周
- 来源宿主：
- Mouse
- 反应种属：
- Bovine
- 应用：
- WB (Western Blot)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
克隆性:Monoclonal
同型:Mouse IgG1
克隆号:N/A
特异性:N/A
纯度:N/A
形式：Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
浓度：N/A
- 说明书：
The S-tag is one of many intrinsically unstructured peptides which only adopts a defined structure on binding to a structured substrate. The S-tag is incorporated into many vectors including the pET29 and 30 series, pCITE-3 and pCITE-4. The S-tag sequence was incorporated into many expression systems and is detectable with certain antibody reagents allowing researchers to check the size and correct expression of recombinant proteins. Proteins including the S-tag can be purified using a column to which is bound the RNAse-S-prot. The modern nomenclature for the enzyme is RNAase A or RNAse 1.
This antibody was made against a synthetic 53 amino acid peptide which is the sequence included in pET30a(+) and other vectors. A C-terminal cysteine was added to allow coupling to maleate activated KLH which was used as the immunogen. Numerous clones were screened by their ability to bind the immunogen and then re-screened for inhibition of this binding by the S-tag peptide. The bovine S-tag sequence is not well conserved across species boundaries, so this antibody is not likely to useful as a general marker for RNAase-1 from other species.
