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Rat CXC-chemokine receptor 3 ELISA Kit
Rat CXC-chemokine receptor 3 ELISA Kit
- 中文名称：
- Rat CXC-chemokine receptor 3 ELISA Kit
- 英文名称：
- Rat CXC-chemokine receptor 3 ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA83375
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|5x96-Strip-Wells|10x96-Strip-Wells
- 保存建议：
- 将所有试剂储存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Rat
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: This CXCR3 ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCXCR3 in the sample, thisCXCR3 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCXCR3 concentration. The concentration ofCXCR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This CXCR3 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCXCR3. Standards or samples are then added to the microtiter plate wells andCXCR3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCXCR3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCXCR3 are added to each well to "sandwich" theCXCR3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCXCR3 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
