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Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppressors Of Cytokine Signaling 3 (SOCS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppressors Of Cytokine Signaling 3 (SOCS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppressors Of Cytokine Signaling 3 (SOCS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppressors Of Cytokine Signaling 3 (SOCS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
