-
Influenza A H5N1 (Avian Flu) Hemagglutinin/HA ELISA Pair Set
Influenza A H5N1 (Avian Flu) Hemagglutinin/HA ELISA Pair Set
- 中文名称：
- Influenza A H5N1 (Avian Flu) Hemagglutinin/HA ELISA Pair Set
- 英文名称：
- Influenza A H5N1 (Avian Flu) Hemagglutinin/HA ELISA Pair Set
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA257808
- 规格：
- 5 Plates|15 Plates|2x15 Plates|3x15 Plates|4x15 Plates
- 保存建议：
- Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Influenza (flu) is a viral respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of mammalian and avian species and is associated with the major human pandemics. Influenza B is largely confined to humans and became unexpectedly prevalent in humans during 2000-2002. Influenza C infects humans, dogs and pigs and generally causes only mild upper respiratory tract infection. However, influenza A and B viruses cause a wide spectrum of severe disease including lower respiratory, tract infection, pneumonia and encephalitis. Influenza A is further divided into subtypes based on antigenic differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). 16 HAs (H1-H16) and 9 NA (N1-N9) had been identified. While different combinations of the two antigens appear more frequently in some groups of birds than others, only few subtypes have established themselves in humans (HA:H1, H2, and H3; NA: N1 and N2). Sustained, widespread highly pathogenic avian influenza (HPAI) H5N1 epidemics represent a significant public health hazard because they not only cause mortality in poultry but also increase the risk of a human influenza pandemic. In 1997, the first case of human infected H5N1 was reported in Hongkong, China. Avian influenza have broken the species barrier and made heavy threaten to human health. In the process of epidemic, more than 100 countries had found more than 400 human avian influenza infected cases, within 262 patients dead. Since two human-to-human transmission cases were reported in China, controlling the interspecies transmission of avian influenza is of critical importance. At least 4 major antigenic groups of H5N1 viruses currently in circulation have caused infection in humans. The recent emergence and development of the unique antigenic FJ-like virus (clade 2.3.4) in Asia, the continuous circulation and expansion of Qinghai-like virus (clade 2.2) in Europe, the Middle East, and Africa, and the persistent prevalence of clade 2.1 viruses in Indonesia, and classic Clade 1 in Vietnam and Hong Kong. Hemagglutinin (HA), which binds to sialic acid (SA)-containing receptors on host cells, is the protein that produces neutralizing antibodies. Hemagglutinin plays a major role in the determination of host range restriction and virulence because human influenza HA preferentially binds to SA-alpha-2,6 while avian influenza HA preferentially binds to SA-alpha-2,3. The cleavage of HA into two disulfide-linked subunits, HA1 and HA2, is a prerequisite for initiating infection. Usually HA is restricted to be cleaved at respiratory tracts by limited proteases. Highly pathogenic avian influenza contains a stretch of basic residues adjacent to the HA cleavage site, enabling its HA to be cleaved by a wide range of proteases with ubiquitous tissue distributions. This process permits productive virus replication in organs outside of the respiratory and gastrointestinal tracts, including the brain, resulting in widespread disease and high mortality rates.
Principle of the Assay: The H5N1 (avian flu) hemagglutinin ELISA Pair Set is for the quantitative determination of H5N1 (avian flu) hemagglutinin. The ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H5N1 (avian flu) hemagglutinin coated on a 96-well plate. Standards and samples are added to the wells, and any H5N1 (avian flu) hemagglutinin present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-H5N1 (avian flu) hemagglutinin monoclonal antibody is then added, producing an antibodyantigen- antibody ""sandwich"". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of H5N1 (avian flu) hemagglutinin present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Intended Uses: The H5N1 (avian flu) hemagglutinin ELISA Pair Set is for the quantitative determination of hemagglutinin. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
