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Rat TNF alpha ELISA Set
Rat TNF alpha ELISA Set
- 中文名称：
- Rat TNF alpha ELISA Set
- 英文名称：
- Rat TNF alpha ELISA Set
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA58620
- 规格：
- 5x96 Wells|10x96 Wells|15x96 Wells|20x96 Wells|
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Intended Uses: The rTNFalpha ELISA Set is intended for use in a 'do it yourself' solid phase sandwich ELISA for the in vitro qualitative and quantitative determination of rTNFalpha in supernatants, buffered solutions, serum, plasma samples and other body fluids.
Principle of the Assay: A capture antibody highly specific for rTNFalpha is coated to the wells a microtiter strip plate. Binding of rTNFalpha samples and known standards to the capture antibodies and subsequent binding of the biotinylated antirTNFalpha secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of rTNFalpha present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of rTNFalpha in any sample tested.
