-
Rat Insulin ELISA Kit
Rat Insulin ELISA Kit
- 中文名称：
- Rat Insulin ELISA Kit
- 英文名称：
- Rat Insulin ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA17361
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存，请将微孔板和标准品保存在-20摄氏度，而剩余的试剂可以保存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Rat
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Insulin (INS) is a protein hormone produced by the beta cells of the pancreas. It plays a crucial role in regulating glucose metabolism. INS lowers blood glucose levels by promoting the uptake and utilization of glucose by cells, and by promoting the storage of glucose in the liver and muscles. It also promotes the synthesis of proteins and fats, and inhibits the breakdown of proteins and fats, to maintain a balance of nutrients within the body. Insufficient levels of INS can lead to diabetes. Type 1 diabetes is caused by an absolute deficiency of insulin, usually due to the immune system attacking and destroying beta cells in the pancreas. Type 2 diabetes, on the other hand, is caused by insufficient insulin or insulin resistance, and is often related to obesity, lifestyle, and genetic factors.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti INS antibody was pre-coated onto the 96-well plate. The biotin conjugated anti INS antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with INS conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of INS in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
