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Human DNA topoisomerase 1 ELISA Kit
Human DNA topoisomerase 1 ELISA Kit
- 中文名称：
- Human DNA topoisomerase 1 ELISA Kit
- 英文名称：
- Human DNA topoisomerase 1 ELISA Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA17592
- 规格：
- 48-Strip-Wells|96-Strip-Wells|2x96-Strip-Wells|3x96-Strip-Wells|4x96-Strip-Wells
- 保存建议：
- 将整套工具短期储存在2-8摄氏度的环境中。如果要长期保存，请将微孔板和标准品保存在-20摄氏度，而剩余的试剂可以保存在2-8摄氏度
- 货期：
- 6-8周
- 检测种属：
- Human
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: DNA topoisomerase 1 (TOP1), an essential manipulator of DNA topology during RNA transcription and DNA replication processes, can prevent R-loop accumulation by removing the positive and negative DNA supercoiling that is made by RNA polymerases during transcription. Diseases associated with TOP1 include Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy 1 and Neutropenia. Among its related pathways are Transport of the SLBP independent Mature mRNA and Metabolism of proteins.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TOP1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti TOP1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TOP1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TOP1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
