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2019-nCoV Spike protein S1 (K417N, E484K, N501Y, D614G)
2019-nCoV Spike protein S1 (K417N, E484K, N501Y, D614G)
- 中文名称：
- 2019-nCoV Spike protein S1 (K417N, E484K, N501Y, D614G)
- 英文名称：
- 2019-nCoV Spike protein S1 (K417N, E484K, N501Y, D614G)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA73332
- 规格：
- 0.01 mg|0.02 mg|0.05 mg|0.1 mg|2x0.1 mg
- 保存建议：
- Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Recombinant protein stored in 50mM sodium phosphate, pH7.5, 300mM NaCl, 150mM imidazole.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Recombinant 2019-nCoV Spike protein S1 (K417N, E484K, N501Y, D614G)(16-685) was expressed in CHO cells using a C-terminal His-tag. The gene accession number is MN908947.
Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world since 2020 (1). The spike glycoprotein (S) of coronavirus binds to the host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) and facilitate viral entry into the host cell (2). SARS-CoV-2 (501Y.V2 variant) with mutations K417N, E484K and N501Y in the spike protein severely affected regions of South Africa (2). A combination of E484K, K417N and N501Y results in the highest degree of conformational modifications of receptor binding domain of spike protein when bound to hACE2, compared to either E484K or N501Y alone. E484K and N501Y increase affinity of RBD for hACE2 and the charge switch due to E484K leads to the formation of favorable contacts (3).
