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Anti-nNOS Antibody
Anti-nNOS Antibody
- 中文名称：
- Anti-nNOS Antibody
- 英文名称：
- Anti-nNOS Antibody
- 品牌：
- phosphosolutions
- 品牌介绍：
- PhosphoSolutions公司成立于2001年。Michael D. Browning, John W. Haycock和Andrew J. Czernik，他们都在Paul Greengard博士的实验室工作过，Paul Greengard博士是2000年诺贝尔医学奖的共同获得者因为他在神经系统信号转导中蛋白质磷酸化的作用。这一专业知识使我们主要关注神经科学领域感兴趣的蛋白质。
- 货号：
- 24121
- 规格：
- 100 µL
- 保存建议：
- Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
- 背景资料：
- Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, [[URL:https://www.novusbio.com/research-areas/neuroscience/neurotransmission.html]][[Caption:neurotransmission]] and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, [[URL:https://www.novusbio.com/common-name/nnos]][[Caption:nNOS]] (type I) and endothelial cell NOS, [[URL:https://www.novusbio.com/common-name/enos]][[Caption:eNOS]] (type III); along with the inducible isoform, [[URL:https://www.novusbio.com/common-name/inos]][[Caption:iNOS]] (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors [[URL:https://www.novusbio.com/common-name/calmodulin]][[Caption:calmodulin]], nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), [[URL:https://www.novusbio.com/common-name/heme]][[Caption:heme]], and [[URL:https://www.novusbio.com/common-name/tetrahydrobiopterin]][[Caption:tetrahydrobiopterin]] (1).
The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and [[URL:https://www.novusbio.com/research-areas/immunology/chemokines-cytokines]][[Caption:cytokines]] such as [[URL:https://www.novusbio.com/common-name/ifn-gamma]][[Caption:IFN-gamma]], [[URL:https://www.novusbio.com/common-name/tnf-alpha]][[Caption:TNF]], [[URL:https://www.novusbio.com/common-name/il-1-beta-il-1f2]][[Caption:IL-1]] and [[URL:https://www.novusbio.com/common-name/il-2]][[Caption:IL-2]]. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and [[URL:https://www.novusbio.com/research-areas/lipid-and-metabolism-diabetes-research.html]][[Caption:type 2 diabetes]]. Acting as a critical mediator of [[URL:https://www.novusbio.com/research-areas/immunology/inflammation]][[Caption:inflammation]] and [[URL:https://www.novusbio.com/research-areas/apoptosis]][[Caption:apoptosis]], iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).
References
1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489
2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174
3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483
- 货期：
- 3-4周
- 来源宿主：
- Rabbit
- 反应种属：
- Human
- 应用：
- WB
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 其他：
推荐稀释比（WB）:1:500-1:1000
Our nNOS rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human nNOS and is antigen affinity purified. It is great for use in WB.
