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PAR2 Peptide
PAR2 Peptide
- 中文名称：
- PAR2 Peptide
- 英文名称：
- PAR2 Peptide
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA76025
- 规格：
- 0.25 mg|2x0.25 mg|3x0.25 mg|4x0.25 mg|5x0.25 mg
- 保存建议：
- Store at -20 degree C for long term storage.
- 货期：
- 6-8周
- CAS号：
- 760-25-8
- 纯度：
- N/A
- 产品形式：
- Antigenic Blocking Peptide
- 分子量：
- 131.55 g/mol
- 分子式：
- C3H8ClF2N
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Overview: Proteinase-activated receptor 2 (PAR2) is a G protein-coupled receptor irreversibly activated by extracellular proteases (1). Activated PAR2 couples to multiple heterotrimeric G-protein subtypes including Gaq, Gai, and Ga12/13 and is rapidly desensitized and internalized following phosphorylation and Beta-arrestins binding. Proteolytic cleavage of PAR2 results in the formation of a new amino terminus that acts like a tethered ligand by binding intramolecularly to receptor to trigger transmembrane signaling. Activated PAR2 also signals independently of Gproteins through its interaction with Beta-arrestins, which promotes sustained mitogen-activated protein kinase (MAPK) signaling, actin remodeling, and cell migration. PAR2 is profoundly localized in the vasculature, especially in endothelial cells, and is implicated in the control of vascular tone and homeostasis. Activation of endothelial PAR2 by endogenous serine protease or by PAR2-activating peptide (PAR2-APs, PAR2 agonist) causes vasodilation in vivo or isolated vessels mainly through nitric oxide (NO) and prostacyclin (PGI2) mechanisms (2).
PAR2 is also expressed in certain types of metastatic cancers and stimulates tumor cell migration and invasion.Vascular PAR2 expression is up-regulated by inflammatory cytokines such as tumor necrosis factor alpha (TNF-a).Increased TNF-a expression in type 2 diabetic coronary arterioles induces activation of reactive oxygen species(ROS), leading to endothelial dysfunction. PAR2 activation promotes cell proliferation in various cancer cell types induding colon, gastric, cervical and pancreatic cancer cells. PAR2 agonists induce Cox-2 expression in lung cancer cells, MMP 2/9 production in prostate cancer cells and vascular endothelial growth factor secretion in breast cancer cells (3). PAR2 is also associated with renal cell carcinoma progression. PAR2 activation has been linked to cancer progression, especially metastasis and angiogenesis as well as pro-inflammatory and anti-inflammatory properties depending on the system. Proteases that are released during inflammation and injury cleave PAR2 on primary afferent neurons to cause neurogenic inflammation and hyperalgesia. PAR2-induced thermal hyperalgesia depends on sensitization of transient receptor potential vanilloid receptor 1 (TRPV1), which is gated by capsaicin, protons and noxious heat. PAR2-induced thermal hyperalgesia depends on sensitization of TRPV1 (4).
The PAR2 selective antibodies were generated against a synthetic peptide taken within amino acid region 300-370 on mouse PAR2 protein. The PAR2 synthetic peptide was covalently modified to achieve desired antigenic properties and was conjugated to a carrier protein before used as immunogen to raise antibodies in rabbits. The PAR2 antibodies are affinity purified over immobilized immunogenic peptide affinity matrix and stabilized with preservatives for long-term storage. Antigenic blocking peptide (P-PAR2) and western blot positive controls (PC-PAR2) are available. Antibodies can be conjugated to fluorophores or secondary enzymes upon request at nominal cost.
