特色产品：

绵羊抗大鼠肾小球基底膜血清（PTX-001AGBM）
Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum
--抗GBM型肾炎模型诱导

 

产品名称	Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum
货号	PTX-001AGBM
规格	25ml
保存	-20℃
说明	产品经56℃热处理30min，请避免反复冻融。解冻后会有轻微沉淀，注射前3000-5000rmp 离心30min。 注：只做过大鼠炎症，没有做过小鼠验证。

应用说明：(实验前请仔细阅读实验流程)

每瓶血清含有足够的抗体用于诱导至少25只大鼠*（175-200 g体重）被动抗GBM肾炎模型。
免疫抗GMB血清：麻醉大鼠，尾静脉注射抗GMB血清超过15秒，疾病的产生依赖于免疫剂量，依照免疫剂量完全打入体内很关键（推荐用量：0.4-0.5ml/100g体重）。由于不同批次抗血清差异，大鼠来源，以及实验要求的不同，我们建议进行剂量-反应研究，具体的免疫剂量和疾病的严重程度，需要根据每个不同批次血清和产生疾病的严重程度，选择合适的免疫剂量。

肾病研究：

1）异源性疾病：免疫抗GBM (anti GBM)3-5天后，免疫荧光检测显示绵羊IgG在肾小球基底膜内呈线状沉积。大鼠c3也呈线性分布，24小时后出现蛋白尿。
2）自身疾病：免疫抗GBM抗体8-10天后，检测大鼠蛋白尿增加，同时大鼠肾小球IgG(自身抗体) 呈线状沉积，自体疾病变得明显。（自体）免疫组化呈线性模式的IgG（图1）。到3周时，蛋白尿有望达到200毫克/24小时，肾小球病变（图2）明显，随着时间的推移，严重程度增加，导致肾小球硬化。

图1.免疫荧光检测显示免疫抗GBM3周后肾小球毛细血管壁大鼠IgG免疫沉积的。

图2.免疫组化染色显示免疫抗GBM 3周后新月体形成、系膜细胞增生、毛细血管变形。

相关产品：

货号	产品名称	规格
肾炎模型
PTX-001AGBM	Sheep Anti-Rat GBM Serum	25ml
PTX-002S	Sheep Anti-Rat Fx1A Serum	25ml
PTX-003S	Sheep Anti-Rat Thy-1 Serum	20ml
PTX-000S	Sheep Non-Immune Serum	25ml
组织产品（动物疾病模型组织、切片、血浆、尿液）
PTX-001	Anti-GBM (Immune-mediated Glomerulonephritis)	-
PTX-002	Anti-Fx1A (Heymann Nephritis, Membranous Nephritis)	-
PTX-003.	Anti-Thy-1 (Mesangial Proliferative Glomerulonephritis)	-
PTX-004	Diabetic nephropathy (streptozotocin- Type I Diabetes)	-
PTX-005	Control (Normal) Kidney	-
PTX-006	Sheep Kidney Section(anti-GBM and Fx1A membranous nephropathy	-
细胞
CRGMes-1	Rat Glomerular Mesangial Cells	1x106
CRGEp-1	Rat Glomerular Epithelial Cells	1x106
CBGEn-1	Bovine Glomerular Endothelial Cells	1x106
CMUB-1	Mouse Ureteric Bud Cells	1x106
CMMM-1	Mouse Metanephric Mesynchymal Cells	1x106
CRMM-1	Rat Metanephric Mesynchymal Cells	1x106
抗体
BS-001GBM	FITC- Sheep Anti-Glomerular Basement Membrane IgG	0.5ml
BS-002ECM	FITC- Sheep Anti-Extracellular Matrix (ECM) IgG	0.5ml

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