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Alanine aminotransferase assay kit (Reitman-Frankel method)
Alanine aminotransferase assay kit (Reitman-Frankel method)
- 中文名称:
- Alanine aminotransferase assay kit (Reitman-Frankel method)
- 英文名称:
- Alanine aminotransferase assay kit (Reitman-Frankel method)
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA172733
- 规格:
- 50 Assays|100 Assays|2x100 Assays|3x100 Assays|4x100 Assays
- 保存建议:
- Store at 2-8 degree C. Valid for 12 months
- 货期:
- 6-8周
- 检测种属:
- N/A
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
Principle of the Assay: Affinity-purified antibody to vWF is coated onto the wells of a microtitre plate. Any remaining binding sites on the plastic wells are blocked with an excess of bovine serum albumin. The plates are washed and plasma or other fluids containing vWF are applied. The coated antibody will capture the vWF in the sample. After washing the plate to remove unbound material, a peroxidase conjugated second antibody to vWF is added to the plate to bind to the captured vWF. After washing the plate to remove unbound conjugated antibody, the peroxidase activity is expressed by incubation with o-phenylenediamine (OPD). After a fixed development time the reaction is quenched with the addition of H2SO4 and the colour produced is quantified using a microplate reader. The colour generated is proportional to the concentration of vWF present in the sample.