-
Low-density lipoprotein cholesterol(LDL) assay kit (Microplate Reader and Biochemical Analyzer)
Low-density lipoprotein cholesterol(LDL) assay kit (Microplate Reader and Biochemical Analyzer)
- 中文名称：
- Low-density lipoprotein cholesterol(LDL) assay kit (Microplate Reader and Biochemical Analyzer)
- 英文名称：
- Low-density lipoprotein cholesterol(LDL) assay kit (Microplate Reader and Biochemical Analyzer)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA172734
- 规格：
- 96 Tests|2x96 Tests|3x96 Tests|4x96 Tests|5x96 Tests
- 保存建议：
- Store at 2-8 degree C. Valid for 12 months
- 货期：
- 6-8周
- 检测种属：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: Lipoproteins (except LDL) such as HDL, CM, and VLDL change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color development. At this time, the LDL particles are still intact, and then the reagent containing coupling agent is added, which can dissociate the LDL particles to release cholesterol, which is catalyzed by cholesterol esterase (CE) and cholesterol oxidase (CO) and produce hydrogen peroxide. Hydrogen peroxide is catalyzed by oxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound. The coloured substance have a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and the LDL-C content in the sample can be calculated.
Intended Uses: This kit can be used for detection of low-density lipoprotein cholesterol (LDL-C) content in serum, plasma, cells, culture supernatant and tissue samples.
