Synthetic peptide taken within amino acid region 175-225 on human CXCR3 protein.
Chemokines (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hematopoietic cells. Chemokine receptors (CXCR) belong to the super-family of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and proliferation and migration of immature neurons, glia and their precursors (1). Furthermore, chemokine receptors articipate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases.
Activation of naive T cells by specific antigen and cytokines, up-regulate cell adhesion molecules (CAM) as well as chemokine receptors on their surface, which directs them to migrate towards the inflamed tissues. The CXCR3 receptor protein is approximately 40-45 kDa protein which has 7 transmembrane domains, characteristic of G-protein coupled receptors. The protein has putative N-glycosylation sites near the extracellular N-terminal end of the protein. The protein has a large 3rd intracellular loop which nteracts with G-proteins. The short carboxyl terminal is intracellular and has putative post-translational sites.
The Anti-CXCR3h-selective antibodies were generated against unique peptide sequence from the CXCR3 receptors gene that is expressed only in a CXCR3 receptor subtype of the human protein. The polyclonal antibodies were affinity purified on an immobilized antigen based affinity chromatography. Antigenic blocking peptides (MBS5401341) and western blot positive control (
MBS542665) in ready to use SDS-sample buffers are available. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost.
